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KMID : 0861020170320030001
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Korea Journal of Herbology
2017 Volume.32 No. 3 p.1 ~ p.7
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Effect of Scrophulariae Radix buergeriana Water Extract on the Proinflammatory Mediators in RAW 264.7 Cells Induced by LPS
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Han Hyo-Sang
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Abstract
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Objective : The purpose of this study was to investigate the effects of Scrophulariae Radix Water Extract (SR) on the production of inflammatory mediators in RAW 264.7 mouse macrophages cells induced by lipopolysaccharide (LPS).
Method : We examined effect of Scrophulariae Radix Extract on the cell viability of mouse macrophages cells. Futhermore, After 24 hours treatment we investigated anti-inflammatory effect of Scrophulariae Radix Extract by the production of Bio-Plex cytokine assay, concentrations of various cytokines such NO, interleukin(IL)-1α, IL-3 and interferon inducible protein-10(IP-10).
Result : No significant changes have been found in the mouse macrophge cell viability by the Scrophulariae Radix Extract at the concentration of 25, 50, 100 and 200 ㎍/㎖. The water extract of Scrophulariae Radix significantly inhibited the production of NO in the LPS-induced macrophage at the concentration of 25, 50, 100 and 200 ㎍/㎖. The water extract of Scrophulariae Radix significantly inhibited the production of IL-1α, IL-3 and IP-10 in the LPS-induced macrophage at the concentration of 50, 100 and 200 ㎍/㎖.
Conclusion : The water extract of Scrophulariae Radix significantly inhibited the production of NO, IL-1α, IL-3 and IP-10 at the concentration of 50 ㎍/㎖ or higher in the LPS-induced macrophages with no changes in the cell viability of them. These results suggest that water extract of Scrophulariae Radix has anti-inflammatory effect related with its inhibition of proinflammatory cytokines such as IL-1α, IL-3 and IP-10 in the LPS-induced macrophages.
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KEYWORD
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Scrophulariae Radix, macrophage, anti-inflammatory effect, cytokine, nitric oxide
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